There is a SA13 human hybridoma cell line in our lab, which - according to the rules of working with cells - is grown on a special IMDM medium. We do not have the opportunity to obtain this medium, but there are DMEM, RPMI, EMEM, alphaMEM media. Can I replace the IMDM with the ones above? Is there a critical difference?
Thanks in advance!
Hello Oleg A. Kuchur
IMDM is a modification of DMEM containing additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. It is used for a variety of hybrid cells. I am attaching the formulations for both IMDM and DMEM. You may compare yourself and find the difference between the two. I would suggest you go for IMDM.
IMDM
https://cdn.stemcell.com/media/files/pis/10000001009-PIS_02.pdf
DMEM
https://www.sigmaaldrich.com/IN/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/dulbecco-modified-eagle-medium-formulation
Best.
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