Dear colleagues, good day!
I have a large ND2 file (~6 GB) that cannot be opened in FIJI due to its size (FIJI says that there is no available memory). I have already increased the available memory in FIJI (up to 100 GB, and surely I always restart the program) without any success. As well, the installation of Nikon ND2 Reader ImageJ plugin (https://imagej.nih.gov/ij/plugins/nd2-reader.html) did not solve the issue. My ImageJ version is 1.54f.
I would be thankful so much for any ideas on how to open such large file (I have no access to the original software that was used to create this file).
Best wishes,
Oleg
Just to add some more info - I have Windows 7 64x installed so unfortunately I am unable to use NIS-Elements Viewer (https://www.microscope.healthcare.nikon.com/en_EU/products/software/nis-elements/viewer).
Have you tried checking the "Virtual Stack" checkmark in the import menu?
Hi Alfonso, and thank you for your suggestion! No, I didn't use this option before, it was always off. Surprisingly, if I toggle this option on, FIJI can't open a large 3D stack ND2 file ("Unsupported file format or file not found").
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