Gromacs 2020: how to make distance restrains for 2 atoms in different protein chains?

Sorry, I think it is rather simple problem to solve, but I am stuck on it. I have 2 protein chains, so I have 2 *.itp files for them that are loaded in topol.top. What should I do to use distance...

01 December 2020 1,496 1 View

Different Ct in qPCR with three sets of reference genes primers (each ref.gene is a single copy per genome), with the same template - is it normal?

I am trying to build up 3 standard curves for 3 sets of primers for different reference genes, in order to later use one with the best efficiency. (for SYBR green qPCR, gene copies number...

27 February 2018 8,313 3 View

QPCR with SYBR green - should the amplicons (GOI and reference) be the same length ?

Do I understand right: when running qPCR with SYBR green detection, it is important that two amplicons (GOI and reference gene) should be of the same lenght ? Once It is supposed to be relative...

07 February 2018 4,678 5 View

How do you assess the efficiency/ rate of mutagenesis, when you plate your culture on the selective plate (e.g. canavanine or 5-FOA) ?

After the chemical or UV mutagenesis, the yeast culture is supposed to be plated on the selective medium with canavanine or 5-FOA in order to assess the efficiency of mutagenesis. The questions...

04 January 2018 4,813 3 View

How to do non-water solvent parametrization for Molecular Dynamics?

Currently I need to parametrize some simple F and Cl containing solvents to use them in Gromacs. The problem is that common force fields do not contain atom-types I need, so while understanding...

18 October 2016 7,283 3 View

Could I expect any quantitative difference in EDX results, if the analysis is performed on SEM/TEM samples (same steel and area)?

INCA (Oxford Instruments), X-Max 80 Cr-Ni stainless steel

16 May 2016 7,436 9 View

How to solve the structure of a complex in crystallography?

22 February 2016 2,615 3 View

Could anyone help me with continuous casting modelling in Abaqus ?

11 October 2014 246 1 View

Diabetes data set for ANN

Hello. Can anyone help me with some useful data for my Master's diploma? I am making an Artificial Neural Network for prediction diabetes. I know about some open data sets like Pima Indians,...

01 January 1970 6,480 2 View

How to avoid fake journal articles published under your authorship without your permission?

01 January 1970 726 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View