In addition, is it better to use plastic or glass tubes?screw cap or cork cap?

Thanks

The best tube is Greiner bio one for cetrifugation for all aplications ecept you need glass then I prefer Erlenmeyer-Kolben. For culture fill only for 1/3 and put it on a shaker in the breeding-room and close it with aluminium folie.

Nice greetings from lower austria

Adequate aeration in almost all experiments with bacteria can be provided by simply leaving the tube's cap (screw cap) slightly loose and culturing in a shaking incubator for 150-250 rpm. And I don't think that the type of tube affect the experiment, you can use the plastic falcon or glass tubes.

Dear Abdurrahaman Aygül!

I am shure that type of tube effects experiments. Fungus they grow on platic tubes and adhere. Some bacteria metabolite plastic..... I had the experience that glass tubes, which was not good cleaned, had substances that inhibit the growing of bacteria...

It is important to know the media and the bacteria to give an good answer! Some bacteria are lipophil too. Is it a secret??

Nice greatings from Bad Vöslau

Heidelinde Schranz

Dear Heidelinde,

Firstly, using not good cleaned tubes is already wrong, namely, let alone your tube contains foreing substances, it must be sterile. The word 'sterile' means, in the general sense, "totally clean".

Besides, I really would like to know which commonly studied bacteria metabolite plastics! Silvia asked a general question without mentioning a specific bacterium, which I agree with you that the question could be more clear, and I added my answer taking into account the frequently studied agents.

And you are talking about fungi, watch that her question is about bacteria.

Lastly, everyone appreciates and Silvia most probably knows that, the glass tubes (if possible newly supplied and ready to use) are the best choice of instrument in all kind of experiments, free from the familiar problems with plastics. But, not everyone has this opportunity every time, so people seek suitable alternatives.

Thank you...

First the tube must be sterile, but on the other side if you make a PCR it must be also free of inhibitors of polymerase. And first ypu want to do an experiment only for bactericidial and then you may have some other experiements for molecularbiology. RNAse- free, DNA-se-free and free of endotoxins.

Bacteria like lipophile Corynebacteria would like to stay on the side of a plastic tube and also Actinomycetales, which are also bacteria and have the same lipophil reaction.

Some strains of S.epidermidis metabolize plastic also in catheter tips. They are biofilm bilding strains.

And yes, I agree new sterile glass tubes are the best solution if they are sterile and heated over a temperature of more than 100 °C. Sorry I forgot how many degree are good to be free of every inhibitors.

And yes, I forgot that the coast´s are really high by only one use of plastic tubes. But for centrifugation I only use plastic tubes, everything else would be to dangerous to crash! A contamination is not funny.

Thank you for your answer Abdurahaman  Äygül

Nice greatings from  lower austria

Heidelinde Schranz