What transfer conditions should I use to blot a membrane protein of size 82kDa?

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What are the membrane transfer conditions I should use during western blot if i have two proteins of varying sizes?

If i need to do western blot of two proteins of varying sizes separated in the same gel (one 55kDa and one 13kDa), what should be the membrane transfer conditions that I need to use? Is 70V for 2...

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Does the use of Phos-Tag give a high background?

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How can I solve my protein instability so it can be used in NMR and ITC studies?

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Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

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Can I use a HisTRAP column for affinity chromatography?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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What is the best blank for nanodrop if I want to read a recombinant protein concentration?

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Can i use the protease inhibitors during cell membrane vesicle preparation ？?

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

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Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

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Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

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