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Questions related from Nisha Rathor
I have cloned a gene in pET28a vector, and induced it for 5 hrs with 1mM IPTG. The cells were centrifuged from 1 ml culture (Uninduced and induced both), and the pellet was suspended in 200...
26 August 2023 3,475 1 View
Is there any online tool available to check, if designed primers are suitable to get overexpression of the histidine-tagged protein, cloned in pET28a vector? How to confirm the suitability of...
08 August 2023 8,282 1 View
I am having genome sequence of one bacteriophage. How to characterize, if the genome is of lytic phage or not.
11 August 2020 3,415 3 View
Many authors used 0.1 M solution of ammonium acatate to prepare bacteriophage sample for Transmission Electron Microscopy. Is isit works to precipate the phage particles or something else. Can we...
17 January 2019 4,604 3 View
I am willing to determine whether, one molecule or combination of molecules of interest our is having potential to reduce the number of pathogenic bacteria, present on any surface or not. what...
31 July 2018 9,257 6 View
What is the meaning of ESP in ESP culture system II for Mycobacterium tuberculosis diagnosis
07 April 2016 330 4 View
What precautions one should take to maintain viability of stem cells of adipose tissue, during their isolation
04 April 2016 4,335 4 View
I wanted to test weather a test compound is inhibiting activity of INHA protein of M. tuberculosis or not.please suggest, how i can design the experiment.
12 March 2016 6,776 1 View
I want to purify His-tagged proteins from them using Ni-NTA column chromatography, so that recombinant tagged proteins remain in their active confirmations.
16 October 2014 5,647 6 View
I want to make knockout strain of Mycobacterium tuberculosis, please suggest available methods.
26 January 2013 3,527 0 View
What is the significance of . in MASCOT as in R.RVSKPGLR.V R is followed by a . and then a peptide chain
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