Nisha Rathor

6 Questions 13 Answers 0 Followers

Questions related from Nisha Rathor

How to stabilize overexpressed Protein?

I have cloned a gene in pET28a vector, and induced it for 5 hrs with 1mM IPTG. The cells were centrifuged from 1 ml culture (Uninduced and induced both), and the pellet was suspended in 200...

26 August 2023 3,419 1 View

How to know if my primers are suitable to clone a gene in pET28a vector to get histidine tagged protein?

Is there any online tool available to check, if designed primers are suitable to get overexpression of the histidine-tagged protein, cloned in pET28a vector? How to confirm the suitability of...

08 August 2023 8,204 1 View

What is the role of Ammonium Acetate in Preparation of sample of bacteriophage for transmission electron microscopy?

Many authors used 0.1 M solution of ammonium acatate to prepare bacteriophage sample for Transmission Electron Microscopy. Is isit works to precipate the phage particles or something else. Can we...

17 January 2019 4,551 3 View

How to enhance viability of adipose-derived stem cells?

What precautions one should take to maintain viability of stem cells of adipose tissue, during their isolation

04 April 2016 4,291 4 View

Which buffer composition should I use to lyse E. coli cells?

I want to purify His-tagged proteins from them using Ni-NTA column chromatography, so that recombinant tagged proteins remain in their active confirmations.

16 October 2014 5,596 6 View

Methodology to make knock out strain of Mycobacterium tuberculosis.

I want to make knockout strain of Mycobacterium tuberculosis, please suggest available methods.

26 January 2013 3,488 0 View