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I am working on the isolation of bacteriophages against Pseudomonas aeruginosa. From one P. aeruginosa isolate, I obtained two plaques with almost similar morphology. I performed 15 rounds of...
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I have cloned a gene in pET28a vector, and induced it for 5 hrs with 1mM IPTG. The cells were centrifuged from 1 ml culture (Uninduced and induced both), and the pellet was suspended in 200...
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Is there any online tool available to check, if designed primers are suitable to get overexpression of the histidine-tagged protein, cloned in pET28a vector? How to confirm the suitability of...
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Many authors used 0.1 M solution of ammonium acatate to prepare bacteriophage sample for Transmission Electron Microscopy. Is isit works to precipate the phage particles or something else. Can we...
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I am willing to determine whether, one molecule or combination of molecules of interest our is having potential to reduce the number of pathogenic bacteria, present on any surface or not. what...
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What is the meaning of ESP in ESP culture system II for Mycobacterium tuberculosis diagnosis
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What precautions one should take to maintain viability of stem cells of adipose tissue, during their isolation
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I want to make knockout strain of Mycobacterium tuberculosis, please suggest available methods.
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