4 Questions 19 Answers 0 Followers
Questions related from Arseniy Yuzhalin
My gene of interest is lowly expressed. What is the maxium amount of template (ng) one could use for RT-qPCR to detect the gene expression? I used 10 ng with no signal at 40 cycles.
10 February 2021 8,779 7 View
I have been experiencing this problem while IHC-p staining my brain tissues. Staining is frequently uneven with gradient-like staining pattern (around 50% of my slides are affected). This happens...
30 October 2020 8,040 4 View
How many days can I keep deparrafinized IHC glass slides in PBS without affecting my staining quality>
16 January 2020 5,095 0 View
Can one perform a Dunnet`s post hoc test for one-way ANOVA analysis if the distribution is NOT normal?
16 November 2015 1,685 3 View