Arseniy Yuzhalin

4 Questions 21 Answers 0 Followers

Questions related from Arseniy Yuzhalin

What is the maxium amount of template one could use for RT-qPCR?

My gene of interest is lowly expressed. What is the maxium amount of template (ng) one could use for RT-qPCR to detect the gene expression? I used 10 ng with no signal at 40 cycles.

10 February 2021 8,948 7 View

What is the reason for uneven staining pattern during IHC-p?

I have been experiencing this problem while IHC-p staining my brain tissues. Staining is frequently uneven with gradient-like staining pattern (around 50% of my slides are affected). This happens...

30 October 2020 8,116 4 View

How long can I keep deparrafinized IHC glass slides in PBS?

How many days can I keep deparrafinized IHC glass slides in PBS without affecting my staining quality>

16 January 2020 5,154 0 View

Can Dunnet`s post hoc test be used with non-normally distributed samples?

Can one perform a Dunnet`s post hoc test for one-way ANOVA analysis if the distribution is NOT normal?

16 November 2015 1,757 3 View