Prabhadevi Venkataramani

7 Questions 9 Answers 0 Followers

Questions related from Prabhadevi Venkataramani

I have to dock two proteins, the residues involved in the interaction of one of them is known. Which docking software would you recommend to use?

I have two proteins that i need to dock. The surface residues involved in interaction for one of the proteins is known from NMR. For the other protein, I need to predict the interaction interface...

03 June 2016 4,202 3 View

What transfer conditions should I use to blot a membrane protein of size 82kDa?

I need to transfer a membrane protein (size 82.9kDa) onto a PVDF membrane. Can anyone help me with the transfer conditions I need to use for this? Is 70V for 4 hrs enough or should I transfer even...

30 December 2015 2,015 2 View

How do I induce a membrane protein expression in pBAD vector using arabinose?

I have to induce a membrane protein in Pseudomonas aeruginosa cells PAO1 that is cloned in pBAD vector using arabinose. Can anyone help with the conditions I need to use. What is the OD that I...

17 December 2015 8,918 6 View

Can anyone help with troubleshooting alanine scanning mutagenesis?

I am doing alanine scanning mutagenesis using Pfu Ultra turbo DNA polymerase and XL1 blue supercompetent cells. My primers have the 3' overhangs. The annealing temperature I have used is 3C lower...

29 October 2015 2,773 3 View

What are the membrane transfer conditions I should use during western blot if i have two proteins of varying sizes?

If i need to do western blot of two proteins of varying sizes separated in the same gel (one 55kDa and one 13kDa), what should be the membrane transfer conditions that I need to use? Is 70V for 2...

31 July 2014 2,549 5 View

Does the use of Phos-Tag give a high background?

I will be incorporating Phos Tag in my protein gels to study histidine phosphorelay between two bacterial proteins. I wanted to know if there is a possibility of getting a high background upon...

17 July 2014 8,580 2 View

How can I solve my protein instability so it can be used in NMR and ITC studies?

I have a highly charged & low molecular weight bacterial protein that is highly unstable. I have tried purifying it with different tags including His tag, MBP tag, Thioredoxin tag, SUMO tag...

09 July 2014 3,835 13 View