The aim of this study was to investigate whether we could detect DNA from pinworm eggs collected from routine diagnostic samples (cellophane tape) and surface-sterilised by hypochlorite. DNA was extracted from individual eggs and tested by PCR
Nooman Siddique
Primers that can be used are: E.vermicu F: 5′-ACAACACTTGCACGTCTCTTC, E.vermicu R: 5′-TAATTTCTCGTTCCGGCTCA.
Additionally you can design one using 5S rRNA gene-IGS region of E. vermicularis, based on your preferences
Hope it was helpful
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Christian G Meyer
you can find a protocol in this paper:
Iñiguez AM et al. Enterobius vermicularis: ancient DNA from North and South American human coprolites. Mem Inst Oswaldo Cruz. 2003;98 Suppl 1:67-9.
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