I want to make a PCR product of a gene that I will use for cloning into expression vector. Since I would like to us a synthetic terminator which is 75 bp long I would need 75 bp long overhang at 3' end of the gene after stop. at 5' end I only need overhang for my restriction site. Do you think is there a problem because the overhangs are not going to be the same length on both sides?

Thank you for your answer!

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