Martin Kavšček

3 Questions 8 Answers 0 Followers

Questions related from Martin Kavšček

CO2 free bioreactor - what are some ways to remove CO2?

We have a putative bacterial strain that we suspect can utilize CO2 as carbon source - As a negative control we would like to grow it in a CO2 free atmosphere on a petri dish - do you have any...

14 August 2015 8,096 6 View

What sould I consider when designing different lengths of 3' and 5' overhangs in PCR?

I want to make a PCR product of a gene that I will use for cloning into expression vector. Since I would like to us a synthetic terminator which is 75 bp long I would need 75 bp long overhang at...

25 March 2015 435 6 View

Yarrowia lipolytica colony PCR protocol?

Dear all, we are working with Yarrowia lipolytica and we have to screen quite some colonies for integration of different genes. We tried the zymolyase protocol to break up the cells and also a...

01 January 1970 593 3 View