I have N-acetylpyrazoline but the purity still 57% that I cannot use it for cytotoxicity assay. I want to recrystallize it but I don't know what solvent I should use. I try methanol and ethanol but it cannot separate my product from another compounds because maybe it has same polarity.
You can recrystallize it from methanol―ethyl acetate (*). Note, however, that N-acetyl pyrazoline may possibly be accompanied by 3,5-diphenyl pyrazoline (**), thus rendering purification challenging.
(*) http://acta.pharmaceutica.farmaceut.org/20306.html
(**) http://pubs.rsc.org/en/content/articlelanding/1952/jr/jr9520004686#!divAbstract
Hi Annisa,
Usually alcohols are good solvent for recrystalization, I do not know exactly what kind of impurity is with your product: any way try the following.
1- use alcohol ( ethanol) to dissolve your compound the add drop wise ether until soln. tern cloudy . then put it in ice bath.
or 2- prepare aqueous soln from your compd. then acidify it with dil HCl, extract with organic solvent ex. CH2Cl2 organic layer put it side. The aqueous layer neutralize it with dil. sodium bicarbonate,then extract using CH2CL2 the organic layer dry it using drying agent then evaporate it, the lifted will be your purified product.
Also do the same on first time extraction organic layer and see what is there.Good luck
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