Hello,
I recently started working with road stormwater runoff and want to analyse the ecotoxicological potential of the leachate and the dissolved particles in a bioassay battery. While testing the native water sample was not so challenging so far, the choice of the right (sequence) of solvents and the suitable method to extract both the metals and the PAHs (and other chemicals) from the leachate seems to be an own field of research. Has anyone around here worked with tire and road wear particles and road runoff so far and has the time to share some of his/her/their experience with me?
Many thanks in advance,
Markus
Dear Markus,
I think these two articles (attached) could actually help you.
Best wishes
Dear Markus, this is an interesting and promising approach. I think your best bet is to use a sequence of SPE sorbents for a broad range of organic (e.g. HLB) and inorganic (e.g. Chelex) chemicals. For the extraction, you can use any suitable (mixture of) organic solvent (e.g. methanol, acetonitrile, acetone, etc.). For the metals, an acid extraction is certainly most suitable. See this paper for organic extraction methods:
https://www.sciencedirect.com/science/article/abs/pii/S0043135418310674?via%3Dihub
Your challenge is going to be to get rid of the acid because it will influence your bioassay responses. To overcome this, we developed a method to get rid of the acid while maintaining the metals in the sample. Check out our paper here:
Article Advancements in effect-based surface water quality assessment
Good luck, and let me know if you have any questions!
Cheers,
Milo
Dear Milo and Mohamed, Many thanks for your help, really good! I will let you know which (combination) of your suggestions and other methods works best
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Pharmaceutical Botany
- Plant Extracts