What is the significance of the following solutions being used as rodent"s organ fixatives, between normal saline, citrate buffer and sucrose?
https://dergipark.org.tr/download/article-file/38655
Can someone explain why the suction pump of the rotary evaporator sucks solvent from the condensation chamber, also pumping out engine oil when it's being switched off and perhaps the solution to...
05 June 2019 1,187 13 View
Must I need to defat the plant sample, before extracting of phenolic rich fraction using the non polar solvent e.g acetone? …
03 April 2019 8,068 9 View
Can someone explain the best way to determine the dose of extract to be administered to experimental animals in evaluating its potency ? Your response will be highly appreciated.
03 April 2019 6,230 3 View
how can qualitative analysis of terpenoid extracted from plants be performed?
02 March 2019 3,324 7 View
Possible and best methods to measure the blood pressure of rats.
02 March 2019 508 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View