how can qualitative analysis of terpenoid extracted from plants be performed?
Take 1 ml of extract of each solvent and add 0.5 ml of chloroform followed by a few drops of concentrated sulphuric acid, formation of reddish brown precipitate indicates the presence of terpenoids in the extract.
Regards
Thanks Mr Nagendra. However, any idea of the possible methods on how to measure the blood pressure of rodents?
Moreover, how do I test for alkaloid and phenolic rich fraction. Regards.
For Alkaloid -1 ml of each extract, 1 ml of marquis reagent, 2ml of concentrated sulphuric acid and few drops of 40% formaldehyde were added and mixed, appearance of dark orange or purple colour indicates the presence of alkaloids.
For Phenols -2 ml of each extract, 2 ml of 5% aqueous ferric chloride were added where formation of blue colour indicates the presence of phenols in the sample extract.
Regards
Dear Fasan Israel, if you want to know in a quantitative way the concentration of terpenes of a plant extract and you use a gas chromatograph coupled to a mass spectrum (GC-MS), you can perform a calibration curve with each of the compounds identified in the GC-MS and thus interpolate in your curve by compound the concentration of each of the analytes present.
I hope it can help you.
Greetings
Sincerely
Cristian Medina
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