I want to use splenocytes as a feeder layer for hybridoma. I want culture splenocytes the day before fusion. I don't know how much cells should be seed in wells?
What challenges do you think businesses or entities face in implementing these strategies? Thanks
28 July 2024 2,125 14 View
Is there any impact of the interest rate, corruption perception index, and economic growth on foreign direct investment in developing countries?
07 July 2024 770 7 View
I injected 28 mycotoxins individually and for 8 of them I did not get ant peaks and for 6 of them the peak shape is not good enough!
29 April 2024 3,641 5 View
Hi everyone, I analyzed some samples by LC-MS/MS, and I got a pretty good peak for quantifier ion, but the peak intensity for qualifier ion is not good enough so I got "ion ratio" as outlier...
21 February 2024 4,249 12 View
Hello, I am trying to remove outliers from a dataset. I removed a few, but they continuously appear. What should I do? Please do not post AI generated answers.
16 January 2024 9,442 4 View
Hi everyone, i have a question regarding my dataset and would be grateful for any help: I have a dataset i downloaded online that gives me information about variables like Pool size, Dealvolume...
07 November 2023 1,005 5 View
Is it possible that TOC analyzing at 680 C cannot measure some organic compounds (e.g. high molecular weight compounds) in oilfield wastewater treatment?
06 November 2023 2,985 0 View
I have an adenovector but the titration is very low and I need more of it.
05 November 2023 3,602 0 View
In the treatment of high salinity oilfield wastewater, we have much higher TOC removal than COD removal.
31 October 2023 965 2 View
I want to design an aptamer for morphine by in sillico method and i need some basic sequence but i can not find them. if you have a library or know some article that can help me , please introduce...
27 October 2023 1,336 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...
09 July 2024 6,336 1 View
I need a copy of the research titled “An Efficient Automated Multi-Modal Cyberbullying Detection Using Decision Fusion Classifier on Social Media Platforms" An efficient automated multi-modal...
08 July 2024 7,381 3 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....
17 June 2024 1,649 0 View
how do integrate ECG, PCG, and clinical data to apply early fusion multimodal?
10 June 2024 3,289 1 View
Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?
20 May 2024 8,214 2 View