What should be the number of splenocytes per well in 96- well plates for feeder cells of hybridoma process?

24 August 2020 0 9K Report

I want to use splenocytes as a feeder layer for hybridoma. I want culture splenocytes the day before fusion. I don't know how much cells should be seed in wells?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Nasim Hafezi's questions See All

How can I magnify an image without changing the dimension in matlab?

Hi everyone. For the last part of my project I have to see how does my program respond to a magnified image. The magnification has to be a part of the code . I've tried imresize() , it will be...

10 February 2021 8,279 10 View

What is the main difference between "CD105+ Endothelial Cells" and mesenchymal stem cells?

since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf? thanks a lot

03 February 2021 5,362 2 View

How to know initial values for non-linear regression?

Does anyone know a reliable methodology to estimate the initial values of theta1 (or A) and theta2 (or B) in a non-linear model? For example, a = (A+Bx/hc)(hc/x-Eg) How to reliably estimate the...

17 January 2021 1,327 1 View

How can i get AMPS-1D resources?

I am trying to model a photovoltaic device using AMPS-1D software. But once I am submitting it to queue, it remains there for infinite amount of time without giving the result. I couldnt...

11 January 2021 1,829 1 View

How can I omit some special part of an image in matlab?

Hi I'm trying to edit my input image which is a simple Persian letter. I want to omit the dots of the letter. I can do it with Photoshop of course, but it would be more technical if I could do it...

14 December 2020 201 5 View

How can I produce hyper immune mouse against human FZD7?

My thesis about the production of mouse anti-human FZD-7 monoclonal antibody (against the 33-185 AA of FZD7) by the hybridoma method. I have a problem with mouse immunization by FZD7. In ELISA the...

10 October 2020 3,741 2 View

How can I design Vanderlugt filter in Matlab?

Hi everyone I need some help in designing Vanderlugt filter in Matlab. I want to design this filter and later use it in a 4f optical correlator. As I tested my designed filter and I'm not sure...

19 September 2020 8,662 7 View

What is the best way to show my live data as an online dashboard using Python?

Hello, I have plenty of data coming from my sensors every minute and I analyze them in Python. I want to make a realtime online dashboard to make a nice representation of my data. My ultimate...

28 May 2020 1,149 3 View

What is the best practice to implement machine learning on real world sensor data?

Hello, I have a few sensors in a home. I am going to continuously receive data fro these sensors, and do Machine Learning (in Python. Keras) on stored data every 24 hours. One possibility to do...

20 May 2020 9,285 7 View

Is there an open-source IoT platform to do online Machine Learning for free?

I am going to connect my sensors to an IoT platform to receive their data and store. Then I need to do machine learning predictions (as Python script) every 24 hours to do predictions for the next...

20 May 2020 590 5 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 9,634 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What is the best technique for combining images?

Hi, Sorry I don't have much experience in image processing. In my research, I need to combine 2 grayscale images(like attached images) into one! I came across the image fusion process. In the...

16 February 2021 7,317 7 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Buillding 3D Model Fussion Protein with Modeller?

Hello May i ask you about using Modeller for making 3D model fussion protein. I want to build a fusion/chimera protein structure which is including 2 different protein and then evaluate quality...

09 February 2021 9,369 2 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does the power law behavior can be approximated with Gaussians?

In epidemiology, earthquakes, tokamak disruptions etc., there is possibility of approximations with the sequence of Gaussians and with the appropriate risks ( see my papers in Journal of Fusion...

26 January 2021 5,411 5 View

Orbitrap Fusion FT keep getting disconnected?

Hi, I been having a problem with the Orbitrap Fusion for quite a while and it has been getting more and more frequent, which is very frusttrating. The fusion FT would get disconnected suddenly...

22 January 2021 1,235 4 View

Sharpening algorithm for similar images with different levels of blurring?

Say for arguments sake I have two or more images with different degrees of blurring. Is there an algorithm that can (faithfully) reconstruct the underlying image based in the blurred...

17 January 2021 7,324 3 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View