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Questions related from Nasim Hafezi
My thesis about the production of mouse anti-human FZD-7 monoclonal antibody (against the 33-185 AA of FZD7) by the hybridoma method. I have a problem with mouse immunization by FZD7. In ELISA the...
11 October 2020 3,884 2 View
I want to use splenocytes as a feeder layer for hybridoma. I want culture splenocytes the day before fusion. I don't know how much cells should be seed in wells?
24 August 2020 9,269 0 View
Hello. I wan to evaluate the function of my protein in MTT assay, I have many problems with the aggregation of this protein after purification. I purify this protein with the IMAC system by GE...
13 July 2020 3,844 0 View
I am working on monoclonal antibody production. I use the SP2/0 cell line. 5 days after fusion I change the medium with fresh fusion medium in HAT, after 6 days I see one or more clusters of cells...
19 January 2020 2,834 2 View