Nasim Hafezi

4 Questions 2 Answers 0 Followers

Questions related from Nasim Hafezi

How can I produce hyper immune mouse against human FZD7?

My thesis about the production of mouse anti-human FZD-7 monoclonal antibody (against the 33-185 AA of FZD7) by the hybridoma method. I have a problem with mouse immunization by FZD7. In ELISA the...

11 October 2020 3,885 2 View

What should be the number of splenocytes per well in 96- well plates for feeder cells of hybridoma process?

I want to use splenocytes as a feeder layer for hybridoma. I want culture splenocytes the day before fusion. I don't know how much cells should be seed in wells?

24 August 2020 9,270 0 View

What is the safe concentration of imidazole for mammalian cells?

Hello. I wan to evaluate the function of my protein in MTT assay, I have many problems with the aggregation of this protein after purification. I purify this protein with the IMAC system by GE...

13 July 2020 3,845 0 View

I have several questions about monoclonal antibody production by hybridoma?

I am working on monoclonal antibody production. I use the SP2/0 cell line. 5 days after fusion I change the medium with fresh fusion medium in HAT, after 6 days I see one or more clusters of cells...

19 January 2020 2,834 2 View