I'm working on magnetic nanoparticle mediated drug delivery. I'm not getting drug release may be because of any error or buffer problem. Anyone please tell me the molarity of buffer PBS.
How did you load your drug molecules in your nanoparticles? I mean the solubility and adsorption process: physisorption/chemisorption and how about the pH of the dispersion? You can see the following:
https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/drug-release
Article Controlled drug release behavior of metformin hydrogen chlor...
Drug is loaded by physiosorption and pH is neutral. But during drug releasing experiments drug is not releasing from nanoparticles. May be due to PBS. Do u know the molarity of PBS to be used in these experiments.
See the following links: If the drug is non-polar, you may have to use surfactant in the release medium.
https://www.thebalance.com/make-phosphate-buffered-saline-375492
Self-Assembly of Partially Alkylated Dextran-graft-poly [(2-dimethylamino) ethyl methacrylate] Copolymer Facilitating Hydrophobic/Hydrophilic Drug Delivery and Imp...
AKS Chandel, B Nutan, IH Raval, SK Jewrajka
Biomacromolecules
It might also be worth to try doing your experiments in different solutions. For example, maybe consider simulated body fluids, artificial lysosomal fluids, simulated gastrointestinal juices etc. (depending on the specific application of the NPs...this would be 'more realistic' compared to PBS and of higher impact)
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