For Drug release experiments, release can be quantified either by FL or U.V . I'm not getting peaks of my drug in U.V during release experiments?

07 July 2019 1 2K Report

I'm doing drug release in 4.3 acidic pH and 7.4 human physiological pH. I'm quantifying the releasing medium by U.V but i'm not getting any peaks of the drug. Any suggestions?

w.s.l. Boyer

Sorry to ask the obvious, but do you get drug peaks when you directly add it to the medium in the concentrations and temperatures you use for the release test?

In addition to the pH, is the temperature also physiological?

Do you have proper agitation?

Do you have dispersion? (If the particles all clump together, dissolution may be slowed dramatically.)

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