My protein hv only one cysteine.i am using 0.7-1 mg/ml protein concentration,what should be the dye concentration for efficient labelling?please suggest me.i am using 100uM TCEP for reducing the cysteine residue.
Adam B Shapiro
Procedural information for protein labeling can be found in the attached document. It recommends 10-20 moles of labeling reagent per mole of protein.
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Steingrimur Stefansson
Hi Shoyab, to increase sensitivity, you could try labeling the protein with streptavidin maleimide and then add biotinylated fluorophores.
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