Shoyab Ansari

6 Questions 8 Answers 0 Followers

Questions related from Shoyab Ansari

Site Directed mutagenesis?

I did SDM.After that i run pcr product on gel and i did get amplification.(Attached pic).Then i did Dpn-1 digestion followed by the gel run again.now this time i did nt get any band and Gel was...

03 March 2019 5,901 5 View

Three bands in Plasmid?

I am doing SDM by stratagene kit.After PCR amplify product was observed on agarose gel.Beside that i run my original plasmid on gel too.Unfortunately after PCR i was not getting correct amplify...

03 March 2019 3,562 8 View

Conjugate concentration after protein labelling?

Quantification of conjugated protein? I am working on protein labelling.I am searching for how to measure the concentration of conjugated protein.(after filtration to remove unconjugated dye). i...

05 May 2018 6,468 0 View

How to prepare Thioflavin stock solution??

how can i prepare thioflavin T stock solution.i have tried methanol,water as well as PBS,,I am not getting any absorption spectra.(which is basically require to calculate the concentration by ex....

05 May 2018 4,798 0 View

What should be the minimum concentration of protein require for enough labelling.I want to labeled cysteine with texas red maleimide. ?

My protein hv only one cysteine.i am using 0.7-1 mg/ml protein concentration,what should be the dye concentration for efficient labelling?please suggest me.i am using 100uM TCEP for reducing the...

04 April 2018 707 2 View

Texas red maleimide in (DMSO) storage and stability ?For how much time i can store dye solution???

texas red maleimide (5mg)-i am using 10mM stock solution.For how many day i can use this dye stock solution??please suggest me?

04 April 2018 1,465 3 View