Three bands in Plasmid?

I am doing SDM by stratagene kit.After PCR amplify product was observed on agarose gel.Beside that i run my original plasmid on gel too.Unfortunately after PCR i was not getting correct amplify...

02 March 2019 3,558 8 View

Site Directed mutagenesis?

I did SDM.After that i run pcr product on gel and i did get amplification.(Attached pic).Then i did Dpn-1 digestion followed by the gel run again.now this time i did nt get any band and Gel was...

02 March 2019 5,897 5 View

Conjugate concentration after protein labelling?

Quantification of conjugated protein? I am working on protein labelling.I am searching for how to measure the concentration of conjugated protein.(after filtration to remove unconjugated dye). i...

04 May 2018 6,464 0 View

How to prepare Thioflavin stock solution??

how can i prepare thioflavin T stock solution.i have tried methanol,water as well as PBS,,I am not getting any absorption spectra.(which is basically require to calculate the concentration by ex....

04 May 2018 4,792 0 View

What should be the minimum concentration of protein require for enough labelling.I want to labeled cysteine with texas red maleimide. ?

My protein hv only one cysteine.i am using 0.7-1 mg/ml protein concentration,what should be the dye concentration for efficient labelling?please suggest me.i am using 100uM TCEP for reducing the...

03 April 2018 704 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

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17 February 2021 4,217 3 View