What should be the minimum amount of water sample for metagenomic DNA isolation from the sample?

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With reference to uploaded paper is it possible to do PFGE with chloroform extraction method or Kit extraction method of DNA?

Also would like to know whether the agarose plugs (after digestion) can be melted to load in the gel for electrophoresis as it is not there in CDC protocol of V.cholerae.

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How do you extract total DNA from water samples for meta genomic study?

I want protocol for marine water sample mostly mangrove

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How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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Leonid Ouchanov

Why don't you try different volumes and come up with the answer yourself?

Smruti Ranjan Nayak

I am yet to do the sampling. so want to know the minimum volumes so that i dnt have to visit the sampling site repeatedly.

Mikhail K Ivanov

You have to know first the concentration of bacteria in the water of your sampling site. It may differ dramatically