What should be the initial pH during measurements of pKa values by Metrohm Titrando-888?

Does the pressure(-600 mmHg) affects pH?

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I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

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Karen A. Darbinyan Popular answer

Hello, The strength of an acid is measured by both its pH and its pKa, and the two are related by the Henderson-Hasslebalch equation. This equation is: pH = pKa + log[A-]/[AH], where [AH] is the concentration of the acid and [A-] is the concentration of its conjugate base after dissociation. What you mean initial pH ? I think doesn't matter .

Karen A. Darbinyan

Mala Babagana Gutti

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Udaya Jayasundara

Can you elaborate your question further? As I understand your question correctly, the initial pH depends on the strength of the acid. If your acid is a strong one, then the concentration can be used to calculate the pH and then it can be compared for the accuracy. If you have a weak acid, then the pKa cones into the picture.