Does anyone have experience in assembling TALEN? Briefly, I'll be doing genome editing to improve crop traits. I have a few questions to clarify before I begin:
1. If I have a complete cds of a gene, do I have trace back to its genomic sequence before I could assemble TALEN? As far as I understand, TALEN only target genomic DNA sequences.
2. If I had to assemble TALEN, is it possible to assemble without having to use kits such as Golden Gate or GeneArt?
3. What are some of the hints/tips for successful assembly?
Thank you in advance.
Short answer:
1: Yes you will have to go back to the genomic DNA, since TALEN target the genomic DNA as you mentioned.
2: It´s possible, but it is a lot of more work!!! you have to assembel various plasmids and it´s no fun at all. I strongly recommend if you wanna stick with TALENS use the CERMAK et al. protocol and stick with Golden gate o.o.
Hope thsi helps,
Cheers
Hi Mr. Thorben Sprink. Thank you for the information. At least my doubts are clarified.
The best advice I can give you if you want to knock out a gene is switching to CRISRP ;)
Cheers
- Badges
- Science topic
- Similar topics
- Biological Science
- Biota
- Eukaryota
- Fungi