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Questions related from Clement K F Wong
Dear All, I'm still relatively new to genetic diversity analysis of plant pathogens. I'd like to ask for suggestions on how to produce a dendrogram consisting of a combination of several genetic...
04 May 2020 7,393 1 View
Good day, all. In a biocontrol study, we can easily determine disease severity by using a disease scale developed for a particular plant disease. On the other hand, disease incidence measures the...
07 August 2019 8,524 4 View
Dear All, I need your help on how to calculate CFU/g soil. I would greatly appreciate if you could check if my calculation is correct. 5 g of soil was placed into 50 mL water. The solution was...
22 April 2019 8,303 8 View
I am interested to study the genetic diversity of a plant fungal pathogen. I have gathered potential genetic markers (around 30 sets) from journals to investigate their % polymorphism before...
10 August 2017 3,670 5 View
I have a question on plant disease sampling methods. I plan to collect wilt samples from different farms. I am rather confuse with the term, "isolates". Does 1 fungi isolate represent 1 farm in...
20 February 2016 1,967 10 View
Does anyone have experience in assembling TALEN? Briefly, I'll be doing genome editing to improve crop traits. I have a few questions to clarify before I begin: 1. If I have a complete cds of a...
25 February 2015 2,820 3 View
I was looking for investigations/papers/researches done on medicinal orchids but the amount of published data on this research seemed disappointing. Are there any reasons behind this?
30 May 2013 5,366 17 View
I've prepared my acetosyringone by dissolving 196.2 mg of it in both 10 ml of DMSO and ethanol separately. Acetosyringone prepared in DMSO precipitated out when I added it into my autoclaved MS...
27 May 2013 9,619 18 View
Are there any methods to transform A. rhizogenes with pCambia 1304? Are these methods similar to transforming disarmed A. tumefaciens?
01 May 2013 3,799 0 View
Currently, my transformed rice seems to have patches of fluorescence instead of spots on its leaves. Is it possible to have this kind of observation? Also, what could be a possible way to evaluate...
03 April 2013 1,745 2 View