What should be an appropriate positive control for intracellular interferon-gamma detection by flow cytometry?

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Do I need specific antibodies to stain for a fusion protein?

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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Why do we equate male and female arousal?

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Senescence-associated beta galactosidase staining is False Positive in control group?

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Suggestions for the Annexin V Positive Control ?

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How can I get clearer background for my western blot?

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Alexis Broquet

Hello, If available in your lab, NK cells treated with IL12.

Otherwise, T cell triggered with a ligand known to induce IFNg should be ok.

alexis

Filippo Veglia

PMA and Iono?

Weisan Chen

PMA+Ionomycin

PHA+Ionomycin

CD3/CD28-dynal beads

Specific antigenic peptides + APC

If you are doing human PAMCs, FEC (Flu, EBV, CMV) peptide pool can be a good one as it covers a good range of HLA alleles