Hello
We want to estimate gut inflammation and oxidative stress. For certain reasons, we can't use gut tissue in human disease model. Can we use stool samples to estimate inflammation cytokines and oxidative stress? Alternatively, I read somewhere that there are few cytokines, which are only associated with gut inflammation and can act as indicator of gut inflammation only. They can be studied in plasma. Are there existing similar oxidative stress indicator for gut as well?
Dear Muhammad,
The following link contains a review article which summarizes all methods to estimate indicators believed to be associated with gut inflammation and oxidative stress in human:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763525/
In addition, the following link may be helpful:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4044300/
Hoping this will be helpful,
Rafik
Dear Colleague
It is a good question and I am afraid that I do not have a proper answer for it. However, I think it would be more practical to run your experiments on intestinal animal models (Organoids) in case human gut biopsies are not available.
I personally used murine bone marrow derived macrophages and infected them with bugs obtained from CD patients. The following link is my recent abstract.
https://www.researchgate.net/publication/305356995_PWE-002_CROHN%27S_DISEASE_MUCOSA-ASSOCIATED_E_COLI_SHOW_BETTER_TOLERANCE_OF_A_SUPEROXIDATIVE_STRESS_ENVIRONMENT_THAT_MIMICS_CONDITIONS_INSIDE_MACROPHAGE_PHAGOLYSOSOMES
Regards
Conference Paper PWE-002 CROHN’S DISEASE MUCOSA-ASSOCIATED E. COLI SHOW BETTE...
HI Dear
it is suitably to use liver biopsy to used them in IHC. the best marker is 8-OHDG. Also you can check them using ELISA kits but the collection and preservation time of serum must be not more than 6 hours.
BR
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus