I would like to simulate a large globular protein of 470 amino acids using replica exchange. I have seen in literature that for larger proteins it is better to use lower transition probabilities than the typical 0.2-0.3. I was wondering why this is and what is a reasonable transition probability for such a large system? I know there are software tools to determine the distribution of temperatures, but what would be a good upper and lower limits for the temperature?

Thanks!

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