What positive controls should I use for indirect ELISA?

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Lora Benoit

use the specific antigen, unlabeled, in solution phase as the positive control

Khadijeh Farrokhi

Lora Benoit dear Lora Benoit my own antigen is biotinylated. I suppose I can not use it as a positive control. am I right? and since I purified my recombinant antibody how can I find the best positive control for that?

Use non biotinylated antigen as a positive control and standard

Lora Benoit Thanks for the help