What Other Methods Are Available For Staining Mycobacteria?

More Abdelazeem Algammal's questions See All

I got this Orcad error (ERROR(ORPSIM-16144): Value may not be 0)?

Dear All I designed the circuit in the attched picture with transformer model T-DYN, but I got this error (ERROR(ORPSIM-16144): Value may not be 0) If anyone cna help Regards The circuit and the...

23 June 2022 8,865 1 View

How to measure EIS for a catalyst?

How to measure EIS for a powder nanocatalyst material ?

05 January 2022 6,425 3 View

Why Inverse Jiles Atherton model does not work like the direct Jiles Atherton model for the same material with the same parameters?

When I made the simulation for the hystressies losses by the direct Jiles Atherton model the resulte were accurate, however when I made it with the inverse Jiles Atherton model the results were...

16 November 2021 7,988 0 View

How to calculate the inductance and resistance of a 'multi-winding transformer MATLAB/Simulink block'?

07 December 2020 8,427 3 View

Are isopropyl alcohol and hydrogen peroxide effective for covid-19?

Are isopropyl alcohol and hydrogen peroxide effective for covid-19? What are the recommended concentration?

20 May 2020 5,059 6 View

What are the internal controls that should be involved in PCR?

13 January 2020 5,105 6 View

What are the best conditions to culture human pulmonary artery smooth muscle cells?

I am trying to start culture with smooth muscle cells isolated form human pulmonary arteries, I would like to know what is the best culture medium to use and the percentage of fetal bovine serum?

21 December 2017 5,814 2 View

What is the best method for tissue sample preparation for the measurement of ADP/ATP ratio using bioluminscence?

I have frozen human tissue samples and I need to measure ADP/ATP ratio in these tissues using a bioluminescence kit from Abcam. what is the best method to prepare my samples without degrading ADP...

07 October 2017 8,453 0 View

Why do some chemists prefer to perform N-formylation for the free amine before coupling reactions rather than reacting the free amine directly?

Recently, I've seen in a patent a simple N-alkylation reaction of 2-aminobenzothiazole with ethyl bromoacetate. The inventors have carried out N-formylation reaction first followed by coupling...

25 March 2015 6,815 7 View

What are the best reaction conditions for the conversion of thiol (SH) group on a heterocycle ring into hydrazide?

I've used excess hydrazine hydrate in ethanol with reflux for more than two days. The reaction is still incomplete and there is a lot of the starting thiol material. Any suggestions?

15 February 2014 3,740 11 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Abdelazeem Algammal

Sputum smears for Mycobacteria can be stained by fluorescent dyes such as Auramine and Rhodamine as they have affinity for mycolic acid In their cell wall . The fluorescent microscopy is useful in screening a large number of specimens. A Large area of smear can be quickly observed that too under high power dry objective.

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC104682/pdf/jm001028.pdf

https://jcm.asm.org/content/jcm/47/4/1206.full.pdf

https://www.ncbi.nlm.nih.gov/pubmed/6180992

Thanks