I have been trying to isolate crude mitochondria from NHF cell lines. Every time I have ended up in different problems. I don't see the mitochondrial pellet or I don't get anything on a western blot when I check.
Maybe one of the methods described in this paper could help you: DOI: 10.5584/jiomics.v1i1.52 Cell fractionation - an important tool for compartment proteomics Maxie Rockstroh1, Stephan A. Müller1, Claudia Jende1, Alexandra Kerzhner1, Martin von Bergen1,2, Janina M. Tomm