What method should be used for analyzing PCR amplification products?

Bassam Yasein Khudaier @Bassam_Khudaier

12 December 2017 0 1K Report

there are many ways for analysis of PCR products, which one is the best

Badges
Science method

More Bassam Yasein Khudaier's questions See All

Is the negative news notification affect human brain psychologically?

life is full of the bad and sad news that may affect people life

04 May 2019 4,952 4 View

To what extent is the boiling method acceptable in PCR and biotechnology applications?

Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology

09 October 2018 705 0 View

What is contig mapping?

DNA consist of series of regions coded for the whole genome.

05 June 2018 4,777 0 View

What is the statement used to describe the process of synthesizing oligonucleotides directly on the glass slide?

There is a process used for synthesis oligonucleotides on the glass slide

05 June 2018 6,207 1 View

How is data mining useful to biotechnology research?

Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.

05 June 2018 4,974 0 View

What makes a bone cell a bone cell as opposed to a liver cell lies largely in the set of genes that the cell expresses?

every somatic cell in the body has basically the same DNA.

04 May 2018 1,441 0 View

What is the role of chloroform in DNA isolation?

chloroform is one of several chemical materials used in the extraction of DNA.

03 April 2018 3,370 2 View

What is the role of Isoamyl alcohol in DNA isolation?

Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.

03 April 2018 8,552 2 View

What is the role of ethanol in DNA isolation?

one of the important steps in the extraction of DNA we use ethanol.

03 April 2018 3,720 0 View

What is the importance of proteinase K in DNA extraction ?

In the process of DNA extraction, we use proteinase K.

02 March 2018 4,461 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View