What medium should I use for cryopreservation of Fusobacterium nucleatum in tissue biopsy taken from colorectal patients?

22 May 2021 0 3K Report

I want to isolate and study the role of Fusobacterium nucleatum (Fn) in colorectal cancer. For cryopreservation of Fn, what medium should I use for preserving the bacteria in biopsy of colorectal tissue until the collection process is completed?

Badges
Science topic

More Goran Qader Othman's questions See All

Can i get some political blogs going?

I'm looking for some blogs It is also possible corpus

14 April 2024 2,090 0 View

How to get AIM for whole sample?

Dear respected Professor and experts in this platform. I would like to ask if anyone here has ever encountered the same problem of getting AIM file, specifically on how to get an AIM file of the...

03 April 2024 2,310 0 View

How to edit preprint article in published article?

When I for the first time made available my the article Legal Aspects of Air Traffic Controller Liability, June 2022, it was in phase of the preprinting. After that when, status of the article...

10 December 2023 252 2 View

Is there a validated questionnaire about caregivers' health-care seeking behaviours for common childhood illnesses ?

Hello everyone, I hope everything is going well with you I'm looking for a validated questionnaire to use in a study to assess caregivers' health-care seeking behaviours for common childhood...

15 September 2023 4,561 0 View

Seeking guidance for validation of a questionnaire?

Dear Academics/expertise I am writing to seek your guidance and validation for the questionnaire that I have developed as a crucial component of my PhD thesis research in management. I kindly...

15 September 2023 8,973 2 View

How to measure current density for electro-oxidation setup with 6 anodes and 7 cathodes?

I have a electrooxidation setup that have 6 anodes and 7 cathodes in alternate arrangement. The surface area of the plate is 1m2 and the applied current is 100A. How to measure the current density...

28 August 2023 164 1 View

How can I avoid the background noise after meusring Floresense with Sytox Green?

I use the HL-60 cell line in conjunction with Sytox Green ( 5µm) to detect extracellular DNA. The cells were suspended in both PBS and complete RPMI. My concern is that the vialable cell group...

06 June 2023 5,301 3 View

In rain modeling by SDSM ????

Hello... In rain modeling by SDSM, there are empty values in the meteorological station file. Should I leave it empty or put a zero? Does it affect the modeling values? and what does the total...

01 June 2023 1,994 2 View

Esclavage moderne : L'être humain dépendant de la technologie! agree or not?

L'apparition des nouvelles technologies de l'information et de la communication (NTIC) a bouleversé le monde entier, et au cours de la dernière décennie, l'être humain a vécu une révolution...

30 May 2023 9,918 0 View

How to rotate a cylinder (geom and results) in comsol?

Hi I am doing a simulation of a cylinder between two walls (2D) using comsol. I divided my simulation into two studies. 1. study 1 (time dependent) the cylinder is at position 1. 2. After...

26 August 2022 5,263 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View