Which heating methods do you recommend for establishing a poultry house (oil heater, gas heater, electric heater)?

I think a gas heater is better for the lower operating costs

03 January 2021 385 18 View

Do you think that the president in Iraq should be able to change the economic situation?

13 December 2020 7,193 3 View

Should I take sample from surface or subsurface for soil analysis?

I am currently doing my final year project on Geochemical analysis of peat samples. I will be looking at parameters such as major and trace elements plus anions and cations. Should i take samples...

27 November 2020 6,668 18 View

#Questionnaire, Big data?

Hello, i'm doing about using big data in telecom companies, but i need a questionnaire to measure big data using, can any one give information about that. thanks a lot, Nechirvan Othman Master...

20 February 2020 4,408 3 View

Saffranin and fast green Staining ?

I need to stain the stem of lily flower (semi-herbaceous). is their any commercial staining that can be used directly. Also, Is their any updated procedure or commercial product (mixture) of...

18 January 2020 3,769 1 View

What is best book to start study charge transport in organic semiconductors?

Could you suggest a good book or review articles on electrical transport and mechanisms in organic semiconductors.

17 December 2018 253 2 View

Can oxalate ion oxidaise V(IV) to V(V)?

Greetings I am engaged with the preparation of VO(II) complexes with the different bidentate amine ligands and the oxalate ion. The starting complex (NH4)2VO(C2O4)2 has been prepared by...

30 August 2018 2,028 6 View

Minirhizotron images data set for root?

Hello, I'm working on olive study and I'm using minirhizotron images to estimate root density in soil profile. However, the image analysis software provide an extra data such as, root surface...

19 November 2017 5,621 3 View

Aquapoinic culture system?

Hello, When we use the aquapponic culture system, does the water that come out from the fish tanks has all nutrients (Macro and micro) that the plant need or should we add some? Thanks

11 November 2017 3,224 4 View

Can we statistically control confounding variables using tests other than ANCOVA or regression?

can we statistically control confounding variables using tests other than ANCOVA or regression? specially in variables that violate parametric assumptions, for example if i am studying the...

22 October 2017 2,896 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View