What marker can I use to sort macrophages from a mixed white blood cell population using flow cytometry??

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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Why activated CAR-Jurkat cell could not kill targets?

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Why do we equate male and female arousal?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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PBMC infection with virus?

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How can I get my Granzyme B flow cytometry stain to be consistent?

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How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

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Jelili Mustapha

Thanks John, that's helpful.

Ann-Kathrin Gaiser

If you would like to differentiate macrophages from monocytes, I would suggest to use CD68.

but be careful. Despite it is a "surface marker", you need to permeabilize the cells for staining because of large intracellular pools.

In order to differentiate between M1 and M2 macs, you might have a look at our publication:

Article Carboxyl- and Amino-Functionalized Polystyrene Nanoparticles...

Good luck!