I've been working on nucleotide analysis by CE. The appearance of a leading peak at about 29min makes me unsure of whether it covers other peaks. So my question is:
1. What makes a leading peak in CE analysis?
2. How to deal with a leading peak to make it more symmetrical to be separated from other peaks?
The details of this analysis are as follows:
CE: Beckman P/ACE MDQ
Capillary: 50μm, 75cm
Optimized conditions: 22 ◦C, 60 mM sodium tetraborate buffer with 1.0% (w/v) PEG polymer (MW= 20,000), pH 9.50.
Any suggestion is highly appreciated.
Hi Dannel, the probable reason would be that, a longitudinal diffusion of your analyte inside a capillary would make the front boundary of injection plug parabolic in shape. This plug when eluting out from the capillary, will show fronting. You can give another pressure impulse after the sample injection which would push the posterior boundary of injection plug and overall the plug will become symmetric.
One of my colleagues recommended me the following paper for this problem:
doi: 10.1021/ac203129h
see if this can work out in your case.
Thank you so much for your very useful information. I'll do some tests and hope it'll work.
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