Rahul D. Dani

4 Questions 12 Answers 0 Followers

Questions related from Rahul D. Dani

Which smoothening model is acceptable for processing of circular dichroism spectra?

For smoothening of noisy CD spectra, several fitting models are available in Jasco spectra manager software, like Savitzky Golay, binomial, means movement and adaptive smoothening. Each one asks...

17 June 2024 4,145 1 View

Is His-Tag by itself immunogenic?

Do we have an evidence that His-Tag related impurities are non-immunogenic? Also, does H6 or H10 get cleaved in the blood stream by exo-peptidases? I want to know the permissible limit of the...

01 November 2023 3,485 1 View

In RP-HPLC runs, I am getting a shift in retention time (peaks are eluting out early by 2 mins), what could be the possible reason?

Mobile phase contains salt and ACN. When same sample is injected again after 3 to 4 different sample injections, the peaks in second run are found to elute out early than first run of the same...

26 April 2016 1,424 13 View

Is perchlorate/ACN mobile phase in HPLC for proteins going to harm their primary structure or is it going to decrease the column life?

There are several papers where people have used perchlorate/ACN combination in HPLC to resolve peptides. However, it is reported that perchlorate in presence of acetonitrile forms free radicals,...

22 April 2016 4,882 1 View