Dannel Wang

4 Questions 6 Answers 0 Followers

Questions related from Dannel Wang

What makes a leading peak in CE analysis?

I've been working on nucleotide analysis by CE. The appearance of a leading peak at about 29min makes me unsure of whether it covers other peaks. So my question is:1. What makes a leading peak in...

26 April 2016 8,200 2 View

What did various signals mean when Waters raw LC-MS data was imported to MZmine2?

Just start to process LC-MS data (.raw) generated by Waters G2S Q-Tof (MSE mode) using a HILIC separation method. A BPI plot was obtained after “Raw data import”, as attached below. I'm...

30 December 2015 854 3 View

Is there any small molecule that could noninvasively decrease the pH of golgi?

It seems that golgi pH could only be increased by small molecular such as Bafilomycin A1; moreover, there are lots of inhibitors that could regulate intracellular pH, but not that of golgi.

09 April 2014 7,333 3 View

How can I measure Golgi pH without transfecting cells with relevant plasmid?

The way of measuring Golgi pH seems to involve fusing a pH-sensitive GFP(pHluorin or deGFP4) with target protein(glycotransferases). Is there any other simpler method such as using a pH-sensitive...

02 March 2014 7,423 4 View