I am working with P6 and P30 rats and I need a loading control to compare and normalize these samples. I have tested actin, tubulin and GAPDH but none of them is constantly expressed between these two stages. Any suggestions?
I doubt that there is a general answer to your question, especially if you do not specify the tissues you work with.
For different stages of development or treatments with global effects I recommend a total protein control such as amidoblack staining once you have finished blotting your membranes.
Are you using brain tissue? have you considered something associated with neurogenesis?
Thank you all for your answers, I am working with skeletal muscle samples (Levator Auris Longus).
I am not an expert on skeletal muscle, but I would try some thing like myosin, a muscle specific collagen, or some nuclear antigen like nuclear pore complex protein, for which excellent antibodies are available.
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