Erica Hurtado

4 Questions 15 Answers 0 Followers

Questions related from Erica Hurtado

It is possible to detect (by WB) a membrane receptor, mainly in the cytosolic fraction after a differential centrifugation?

Hi, I performed differential centrifugation: 3,500 rpm for 15 minutes, and the resulting supernatant was further centrifuged at 45,000 rpm for 1 hour to obtain the cytosolic and microsomal...

12 December 2015 2,501 4 View

Has anyone detected BDNF in tissue homogenates by ELISA?

We are using BDNF kit to detect BDNF in rat muscle homogenate. The standard curve is correct, so the kit is working well, however in our samples have a lot of variability in different...

01 January 2015 7,177 8 View

Anyone have suggestions for a peptide inhibitor or antibody that can binds to BDNF or NT-4?

We want to inhibit the binding of BDNF and NT-4 to its receptor. So we are looking for some peptide inhibitor or antibody that can binds to these proteins in their binding sites. Any suggestion?...

02 February 2014 9,803 10 View

What loading control in Western Blot can I use to compare different stages of development?

I am working with P6 and P30 rats and I need a loading control to compare and normalize these samples. I have tested actin, tubulin and GAPDH but none of them is constantly expressed between these...

09 September 2013 7,563 5 View