Hello, I am just getting started on a project to look at phenotypical characteristics of M1/M2 macrophages, and I'm not sure if I should head toward working with human PBMC/monocytes or find a good mouse cell line like J774A or RAW264.7. I am unsure of what to take into account. Any tips/experience would help. Thank you!
Dear Hallie
Look Mini-review for Macrophage polarize introduce by Bio-Rad Laboratories,inc attached.
may help you.
Good luck
Hello Hallie
I have worked in monocyte and monocyte derived of macrophage. According to my experience, you sholud start by human macrophage and test the phenotype markers for M1/M2 accoriding your conditions. Remember the plasticity of macrophage that not always is reproducible in others cell lines. After, whether you want to do a functional analysis (overexpression - inhibition), may be you should test the performance technic. In contrast, in this step, you could use line cells that express your phenotype profile in human macrophages previously found.
Regards
Hello Oscar,
To start off, I am trying to find a mouse monocyte cell line to do continuous work on. I would like to differentiate them into macrophages eventually, but is it possible to buy a monocyte line and be able to keep a continual source of them without them differentiating? Or will they differentiate still without additional growth factors?
Thank you for your help!
Hi Hallie
yes, I worked with monocyte line as HL60 or THP1 (undifferentiated cell). The first step is increasing the cell concentration and after a subset is used to store (cryoconservation) the cell line and other for the cell differentiation to macrophages with TPA stimulation.
The store is better with undifferentiated cell than cells differentiated to macrophage. Because, criopreservation step affect the concentration cell, but it is compensated in the increasing cell previously to differentiation.
Regards
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