What kind of contamination is this ?

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What basic protocol can you recommend for making liposomes with a highly water insoluble drug?

I am new to liposome making. My drug (carbamazepine, mol wt. 236) is highly insoluble in the water. I tried to load it in bilayer using a basic protocol. After lipids(DMPC, DMPG, Cholesterol)...

23 November 2020 4,886 3 View

Does anyone has experience with Dextran-FITC uptake by cancer cells?

Please share the protocol for antigen uptake assay by cancer cells. I want to use Dextran-FITC to track the endocytosis by cancer cells. If anyone has the protocol or any suggestions kindly share.

25 October 2020 469 3 View

Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

05 November 2019 7,719 2 View

The right statistical test for microbiological data from food production environment?

I am analyzing the safety aspects of two food production sites producing similar products. I wish to compare these geographically distinct sites across seasons as well as processing steps. I have...

15 July 2019 550 3 View

Can anyone please suggest me code of LEACH algorithm in python.?

02 July 2019 5,023 1 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

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27 June 2019 8,798 3 View

Nuclear fractionation protocol for ChIP-sequencing and Co-IP mass spectrometry?

The protein I am working on is a transcription factor and is abundant in nucleus. I want to do subcellular fractionation of the adherent tumor cells followed by ChIP sequencing in order to...

02 December 2018 5,978 1 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

Please suggest me the method to determine the thickness of coating on whole fruits and vegetables?

How can be the thickness of coating on fresh whole fruits and vegetables be determined?

04 November 2018 4,296 4 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Which database could provide the signal sequence for targeting various cellular regions? ?

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04 February 2021 6,650 2 View

Moving bacteria-like organisms in my HEK293T cell culture?

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02 February 2021 7,892 4 View

I want to extract engine oil from a soil sample,please how much amount of anhydrous sodium sulphate am i going to add to 10kg soil?

Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate

01 February 2021 9,509 1 View

Yunus Shukor

Very similar to

https://www.researchgate.net/post/Has_anyone_seen_this_kind_of_cell_culture_contamination_Do_you_know_what_it_is

Kiprotich Kiptum

Normally U87MG forms these clusters when they are at higher density. The challenge is also is that you might have kept U87MG for longer than 4 days without subculture.