I would like to find an anesthetic that has the least effect on coagulation factor.
Thanks
Among the Intravenous induction agent , i.v propofol @ dose 1mg /kg of body wt of Rat is sufficient for withdrawing blood for cogulation profile test with no effect on coagulation . Ketamine is avoided because of its effects on platelet aggregation.
Hi Alok,
Thanks for your answer and help .
Is there any reference or text that I could use for this?
Yours
Sajad
Hi, study done on effect of propofol on cogulation parameter in Human being is safe Ketamine is not safe for the study on coagulation parameter
This is more complicated that it first appears. There is a powerful link between nervous activity and coagulation via von Willebrand Factor (VWF), which is released in accord with sympathetic nervous activity. VWF binds to enzymatic factor ViIIC from the liver. This creates a gigantic molecular complex called “factor VIII.” VWF stabilizes VIIIC to enable its enzymatic activities. Factor VIIi reacts with factors VII, X and tissue factor to generate factor XIII, which adds “cross-links” to soluble fibrin to generate insoluble fibrin. The insoluble fibrin binds to red cells to increase blood coagulability. Thus, anything that increases sympathetic nervous activity can exaggerate blood coagulability and perturb your results.
To control sympathetic nervous activity, you will need a combination of anesthesia and analgesia. I would suggest that you anesthetize the rats with 1/2 MAC isoflurane supplemented with 10mcg/kg fentanyl. Then draw your blood samples.
Dear Sajad , You can see study of Intravenous induction agent like Etomidate, Thiopentone ketamine and propofol effect on cogulation in human .if it is safe in human , definitely will safe in Rat.because Before the drugs to launched in market should be safely passed in animal model . As far as I know Propofol has nil effects on coagulation
Don't be so trusting of Big Pharma. Propofol is notoriously toxic. It's cardiotoxicity can cause irreversible cardiac arrest and death. Happens all the time. When used as an infusion for prolonged sedation, it can destroy muscle tissue, especially in children. It burns like hell when injected intravenously unless it is mixed with lidocaine. I quit using it soon after it was introduced, but most anesthesiologists love it because it prevents vomiting and nausea, and because they think it is short-acting. Actually, it has a prolonged synergistic interaction with narcotics that causes respiratory depression. Anesthetizing rats with intravenous propofol will almost certainly cause pain, and the pain will increase sympathetic nervous activity, and the sympathetic nervous activity will release von Willebrand Factor from the vascular endothelium, which will increase factor VIII activity and increase coagulability. Etomidate disturbs steroid metabolism somehow and increases morbidity and mortality when used for anesthetic induction in critically ill patients------but anesthesiologists love it because it doesn't immediately decrease blood pressure. I don't use Etomidate, either. I don't know if ketamine affects blood coagulability, but I wouldn't be surprised. If you really want to minimize nervous activity that might exaggerate blood coagulability, I recommend inhalation induction with isoflurane followed by fentanyl supplementation. I know this will be a nuisance, but it should help to eliminate the variable of nervous activity that might otherwise perturb your research results.
Coleman Sir , Propofol is excellent induction agent for day care surgery because of its unique rapid onset and offset property ,it's all happens due to its redistribution phenomenon. Inj propofol + inj fentanyl at appropriate dose will be ideal choice Many study proved that Propofol has no effect on coagulation. TIVA technique ( propofol + fentanyl) will be take care of pain mediated stimulation of Sympathetic surge
I'd like too see if you adhered to that viewpoint after someone induced anesthesia with pure intravenous Propofol------after you stopped screaming, of course. If you use fentanyl to to prevent the screaming, you must administer it a couple of minutes in advance to allow it time to take effect. I wouldn't use Propofol on a dog. It's toxic waste poison. Convenience is no excuse for sacrificing safety. I suppose you also advocate using carbon dioxide for both anesthesia and euthanasia.Such is the state of anesthesia insanity that prevails in practice. Try reading the attached copies of published papers.
At any rate, we can't communicate with rate to learn what they experience. I know from speaking to researchers that rats do not experience the sort of airway obstructions that humans exhibit; whether they are induced with inhalation agents or intravenous opioids they tend to lie down without occluding their airways. So, I would guess that the least stressful way to anesthetize them would be to place them in a box and gradually increase their exposure to isoflurane until they are anesthetized enough to tolerate venipuncturing for blood sampling. That would minimize, but not eliminate, the sympathetic nervous activity induced by venipuncture.
Dears Alok and Lewis, thank you for sharing your knowledge. After reading your vauable comments and reading more on those techniques I found an article and some more interesting, which confirm using both ways. Particularly the attached paper and the following paper that I could not get the full text "Effects of two injectable anesthetic agents on coagulation assays in the rat" are interesting. I decided to go a head with isoflurane inhalation which is more feasible for us.
Thanks again
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