What is it? Subject classification codes?

Dear colleagues, help me to understand the rules for authors. I am writing an article for journal "Climacteric". According to the rules for autors it is necessary to indicate "Subject...

09 February 2021 2,221 4 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

Variable with negative values in SPSS?

Hello, dear colleagues! I would ask you for a little help. Actually, I'm doing a multivariate linear regression analysis in the SPSS and one of the independent variables has negative values. In...

20 December 2020 535 2 View

ICP-Analysis: how can I consider the loss of sample mass after sample filtration?

To prepare a sample for ICP-Analysis one has to dissolve the material in the chosen liquid and then filter it. How can I consider the loss of mass after filtration if I don't know it exactly? I...

20 December 2020 4,394 3 View

How to report results from piecewise structural equation modelling analysis?

I have done my analysis in R using the piecewiseSEM package and have got the results but I am unsure how to report it APA style. What values do I report? Thank you in advance!

13 December 2020 3,048 3 View

Is USDA NASS plot-level Objective Yield Survey data publicly available?

I'm interested in the results of Objective Yield Survey by USDA NASS in a raw form of plot-level observations. As far, as I understand, quickstats...

19 November 2020 5,369 3 View

What is the most sufficient way of protein immobilization on SiO2?

I need to immobilize 3 peptides: CGPDGRDGRDGRDGR, CRQILIWFQNRRMKWKKYQRLC, penetratin peptide RQIKIWFQNRRMKWKKGG on the SiO2-covered gold nanoparticles. What is the most efficient and stable way...

16 November 2020 2,909 3 View

How to dissolve betulin in DMSO (20mM stock solution)?

I would be very grateful for any help regarding my problem. My research requires applying betulin (and betulinic acid) solution on the cerebral cortex slices of rats. With the stock solution of...

27 September 2020 8,758 2 View

Scanning Electron Microscope- How to correct crossover?

Hello, I have questions about XL30 SEM (with tungsten filament). Can anybody help me with how to correct crossover? You will see in the picture look of my crossover. I trying everything and can't...

24 May 2020 1,339 3 View

Is it possible to precisely evaluate a thickness of a NiP-Cr coating on a stailness steel with XRF?

Hello everybody, I have a qestion about the implementation of the XRF to determine the thickness of the NiP -Cr coating on a stainless steel (1.4305). According to the TDS of the base Material,...

24 February 2020 5,574 2 View

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02 March 2021 3,060 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

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28 February 2021 9,936 2 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View