What is this contamination?

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Experts on orchid viruses for an article for home gardeners?

I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about...

26 June 2024 7,422 1 View

Job Evaluation - what is the key advantage / key disadvantage of point-factor rating?

I am look at Job evaluation and it feels that the advantages of point-factor rating seems to also be the disadvantages - for example it is objective, but also subjective

14 May 2024 4,747 0 View

Which questionnaire can be used to investigate which leadership style an employee prefers?

For my thesis, im doing research in finding the leadership style that is the best match with organization X to maintain work engagement. For this research I'm going to interview 10 employees and...

19 March 2024 539 0 View

Who is researching trauma right now, specifically childhood trauma?

Hi! I am graduating with my masters in Marriage & Family therapy the end of February & want to continue my education with a PhD. I applied to Florida State but that did not work out. What...

07 February 2024 3,341 2 View

Which LAMP kit works better: NEB or ThermoFisher?

Hello, I'm currently in need of performing Loop-Mediated Isothermal Amplification (LAMP). There are modifications in both the Bst DNA polymerases from NEB and ThermoFisher. Has anyone had...

31 January 2024 2,534 1 View

What is recent development/algorithms in elliptic curve cryptography for image encryption?

Image and text encryption

26 January 2024 1,223 4 View

Is anyone aware of a set of accessible anonymised free text clinical records related to primary care?

I am looking for access to some publicly (or cheaply) available free-text clinical records, to help with the development of an AI tool. I am aware of CPRD (too expensive, not free text) and...

15 January 2024 7,535 1 View

Prostate Cancer Cell Lines from Black / African origin patients?

Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the...

07 December 2023 4,969 3 View

What type of sample prep is needed for SDS-PAGE electrophoresis of plasma?

I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?

06 December 2023 8,966 1 View

What are evidence-based self-care strategies for psychologists?

Models of self-care Strategies for self-care Trainees Clinicians

25 October 2023 8,634 4 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View