What is the void volume (%) in general in Hydrophobic interaction chromatography?
The void volume depends on the type of resin being used and the volume of the column.
If you are running a HIC chromatography where your target protein is bound by the HIC interaction, there really is no void volume. As you will more likely bind, perform a wash step and then stepwise or gradient elute the HIC media with High Salt Buffer to Low Salt buffer. Depending on the HIC interaction your target protein will elute at some point in the gradient or stepwise mode. If your target protein does not bind, then there really is no void volume to consider as well.
@Vernon: Thanks. Actually we are working on optimization of a flow through HIC wherein our product of interest (a polysaccharide not a protein) does not bind. However it was useful to know void volume to optimize the conditions for wash after loading.
@Heironim: we also think that it should be in the same range as of SEC (~30-35%), wanted to confirm from experts
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