I am trying to simulate (in Comsol) electric field through an electrophoresis system and need an approx. value for the dielectric constant/relative permittivity of any kind of gel (like PAGE or agarose) etc. Any ideas?
For SDS-PAGE it is 100 V for 90 min. You can change the volt from 90-110 and also the time point. but the time should not be less than 90 min.
Is there any research/groups working into miniaturizing AC Pulsed Field Gel Electrophoresis? Like if you want to use it a lab-on-a-chip or similar formats.
04 May 2017 9,609 1 View
What are the best ways to make extruded acrylic sheet surface hydrophobic? I understand Teflon coating can be one way to do it? Is there a recipe for the same? Are there other ways to achieve...
05 June 2016 5,655 5 View
What are the conventional methods of DNA extraction from bacteria (lysis & purification) that would ensure the DNA strands does not fragment during lysis & purification? Is there a...
03 April 2016 4,085 4 View
Is there a biophysical difference between exosome & oncosome in terms of charge, size, surface proteins etc.? I read oncosomes are ~1 um compared to ~100 nm exosomes. Is that always the...
09 October 2015 7,421 2 View
I have been trying to detect DNA hybridization on GCEs using DPV in acetate buffer. But I get a lot of background current. I saw papers (DOI: 10.1021/ac981432j) using baseline correction to...
08 September 2014 3,780 2 View
I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being...
07 August 2013 5,205 6 View
Does the number of layers of deposited graphene oxide thickness determine electrochemical (EC) performance of Glassy Carbon electrode? If you say electrodeposit a thicker layer is that good or bad...
05 June 2013 4,054 9 View
I am preparing Graphene Oxide/GO using Hummers method. The protocol mentions filtering the GO. But that takes a week and it's hard to get the GO flakes off the filter paper. Can we do it faster by...
05 June 2013 1,998 20 View
I am trying to run DPVs using a GCE/glassy carbon working electrode on Ruthenium in 0.2 M acetate buffer (Pt CE & Ag/Agcl RE). When I try using a lower surface area electrode I see that noise...
05 June 2013 5,018 20 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...
02 March 2021 2,431 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...
02 March 2021 9,157 1 View