I am trying to simulate (in Comsol) electric field through an electrophoresis system and need an approx. value for the dielectric constant/relative permittivity of any kind of gel (like PAGE or agarose) etc. Any ideas?
For SDS-PAGE it is 100 V for 90 min. You can change the volt from 90-110 and also the time point. but the time should not be less than 90 min.
Is there any research/groups working into miniaturizing AC Pulsed Field Gel Electrophoresis? Like if you want to use it a lab-on-a-chip or similar formats.
04 May 2017 9,708 1 View
What are the best ways to make extruded acrylic sheet surface hydrophobic? I understand Teflon coating can be one way to do it? Is there a recipe for the same? Are there other ways to achieve...
05 June 2016 5,762 5 View
What are the conventional methods of DNA extraction from bacteria (lysis & purification) that would ensure the DNA strands does not fragment during lysis & purification? Is there a...
03 April 2016 4,185 4 View
Is there a biophysical difference between exosome & oncosome in terms of charge, size, surface proteins etc.? I read oncosomes are ~1 um compared to ~100 nm exosomes. Is that always the...
09 October 2015 7,543 2 View
I have been trying to detect DNA hybridization on GCEs using DPV in acetate buffer. But I get a lot of background current. I saw papers (DOI: 10.1021/ac981432j) using baseline correction to...
08 September 2014 3,895 2 View
I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being...
07 August 2013 5,363 6 View
Does the number of layers of deposited graphene oxide thickness determine electrochemical (EC) performance of Glassy Carbon electrode? If you say electrodeposit a thicker layer is that good or bad...
05 June 2013 4,171 9 View
I am preparing Graphene Oxide/GO using Hummers method. The protocol mentions filtering the GO. But that takes a week and it's hard to get the GO flakes off the filter paper. Can we do it faster by...
05 June 2013 2,305 20 View
I am trying to run DPVs using a GCE/glassy carbon working electrode on Ruthenium in 0.2 M acetate buffer (Pt CE & Ag/Agcl RE). When I try using a lower surface area electrode I see that noise...
05 June 2013 5,175 20 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View