Harikrishnan Jayamohan

10 Questions 32 Answers 0 Followers

Questions related from Harikrishnan Jayamohan

How to minaturize Pulsed Field Gel Electrophoresis?

Is there any research/groups working into miniaturizing AC Pulsed Field Gel Electrophoresis? Like if you want to use it a lab-on-a-chip or similar formats.

05 May 2017 9,665 1 View

Best methods to create a hydrophobic surface on PMMA/acrylic sheet?

What are the best ways to make extruded acrylic sheet surface hydrophobic? I understand Teflon coating can be one way to do it? Is there a recipe for the same? Are there other ways to achieve...

06 June 2016 5,701 5 View

What are the methods of extracting bacterial DNA for long sequencing reads, ensuring DNA does not fragment during the process?

What are the conventional methods of DNA extraction from bacteria (lysis & purification) that would ensure the DNA strands does not fragment during lysis & purification? Is there a...

04 April 2016 4,136 4 View

What are differences in biophysical properties of exosome vs. oncosome?

Is there a biophysical difference between exosome & oncosome in terms of charge, size, surface proteins etc.? I read oncosomes are ~1 um compared to ~100 nm exosomes. Is that always the...

10 October 2015 7,476 2 View

What does the level 2 Savitzky and Golay filter correspond to in terms of polynomial order or frame size in MATLAB?

I have been trying to detect DNA hybridization on GCEs using DPV in acetate buffer. But I get a lot of background current. I saw papers (DOI: 10.1021/ac981432j) using baseline correction to...

09 September 2014 3,845 2 View

What is the value of the dielectric constant/relative permittivity of electrophoresis gel?

I am trying to simulate (in Comsol) electric field through an electrophoresis system and need an approx. value for the dielectric constant/relative permittivity of any kind of gel (like PAGE or...

06 June 2014 2,867 1 View

Is there a particular reason DPV is preferred over EIS or are there any cons or advantages of one over the other?

I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being...

08 August 2013 5,306 6 View

Effect of layers of graphene oxide deposited on GCE (or other substrate).

Does the number of layers of deposited graphene oxide thickness determine electrochemical (EC) performance of Glassy Carbon electrode? If you say electrodeposit a thicker layer is that good or bad...

06 June 2013 4,116 9 View

Can someone help me in regards to separating Graphene Oxide by centrifugation?

I am preparing Graphene Oxide/GO using Hummers method. The protocol mentions filtering the GO. But that takes a week and it's hard to get the GO flakes off the filter paper. Can we do it faster by...

06 June 2013 2,173 20 View

How to reduce noise during differential pulse voltammetry? Effect of working electrode area?

I am trying to run DPVs using a GCE/glassy carbon working electrode on Ruthenium in 0.2 M acetate buffer (Pt CE & Ag/Agcl RE). When I try using a lower surface area electrode I see that noise...

06 June 2013 5,107 20 View