If there is a chromophore group containing terpene in natural extract, for the seperation in HPLC with UV detector, What are the wavelength I can set..?
If you are working with PDA then go for 210 or 220nm.
But for terpenes use ELSD detector
Thanks Jack Silver, this is only example. Can I try to seperate with this wavelength..?
Molecular weight of my compounds are 260,234
220 nm for terpenoids is reported. Please see attached file for details of the method.
Please also refer the following for HPLC details:
Baas, W. J. and Niemann, G. J. (1978), High performance liquid chromatography of terpenoids. J. High Resol. Chromatogr., 1: 18–20. doi: 10.1002/jhrc.1240010105
http://onlinelibrary.wiley.com/doi/10.1002/jhrc.1240010105/abstract
Regards
Base on my experiences, you can use 270 and 290nm for diterpenes and triterpenes. Of course diterpenes have weaker absorption usually.
@ Byju Ponnana: You can use 235 nm if your solvent doesn't absorb at that wavelength. I assume you are using normal phase solvents and most of them absorb in this wavelength range. You may be able to use acetone for the "Strong" solvent as described in this application note:
http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN20_Acetone_as_an_Alternative_to_Ethyl_Acetate.pdf
Briefly, this works because acetone has a "hole" in its absorbance spectrum. If you are using reverse phase solvents (water/acetonitrile, methanol, ethanol), pretty much any wavelength will work.
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